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* T & C
You will get a call within 12 Hrs from the time of Booking. *T&C – Free home sample collection is not available on specific tests. Pediatric home sample collection ( kids below 10 years age ) is available at specific cities only

Vitamin B-12 Test

What is Vitamin B-12?

B-12, also known as cyanocobalamin, is one among the eight different types of the water-soluble Vitamin B, the other seven being thiamine (B-1), riboflavin (B-2), niacin (B-3), pantothenic acid (B-5), pyridoxine (B-6), biotin (B-7) and folate or folic acid (B-9).

When is a Vitamin B-12 test advised?

B-12 deficiency leads to megaloblastic anaemia, nerve impairment, dementia, a tongue condition called glossitis, and a spinal cord disease called myelopathy. It is often associated with thyroid disease and multiple sclerosis. As a result, the following symptoms may be noticed:

Weakness and fatigue

Pale skin

Blurred vision

Heart palpitations


Problems related to nerve damage, such as pins and needles, numbness, tingling, etc.

Mental issues such as depression, memory loss, and confusion.

What causes Vitamin B-12 deficiency?

Vegans and vegetarians are at a disadvantage because of the unavailability of meat sources. Certain conditions and disorders of the body also restrict Vitamin B-12 levels:

Atrophic gastritis, which results in the thinning of the stomach lining.

Pernicious anaemia: Two bodily secretions are involved in the absorption of B-12: hydrochloric acid, which separates the vitamin from the protein that is attached to it, and a protein in the stomach called intrinsic factor, with which it combines to be absorbed by the body. In a condition called pernicious anaemia, the body is unable to produce this intrinsic factor, which reduces its ability to absorb Vitamin B-12 drastically.

Immune system disorders

Small-intestine disorders

In these conditions, tests are recommended.

What are sources of Vitamin B-12?

Vitamin B-12 is found mostly in meats such as poultry, beef, pork, ham, lamb and fish. Dairy products such as milk and cheese are also good sources, as are fortified varieties of soy milk and breakfast cereals.

What are the functions of Vitamin B-12?

B-12 is concerned with the healthy functioning of nerves and the production of red blood cells. It also helps make and regulate DNA in the cells.
Vitamin B 12 is also involved in the metabolism through its role in energy production and synthesis of fatty acids.

The recommended intake level per day is 2.4 mcg (micrograms) for teenagers and adults, 2.6 mcg for pregnant women, and 2.8 mcg for lactating women. For children below 13 years, it varies according to their age.

What is the testing procedure?

B-12 levels can be measured through a simple blood serum test. A normal reading would register between 200 ng/ml and 900 ng/ml. If the reading is less than 150 ng/ml, a methylmalonic acid (MMA) test is advised.

However, the blood serum test measures even unusable B-12, giving an insignificant result and covering up an actual deficiency. On the other hand, measuring the concentration of metabolic products such as methylmalonic acid, whose conversion is dependent on Vitamin B-12, gives a clear result. Thus, a rise in the levels of this acid in the blood or in the urine is a confirmatory test for low B-12 levels. Up to 2mg MMA/g of creatine signifies no deficiency, but a higher value is an indication of deficiency. A simple urine test is sufficient for this purpose.

Another test called HoloTC measures just the biologically available B-12 in the blood, and is therefore more reliable than the blood serum test. It measures the amount of B-12 bound to transcobalamin II and is considered an early warning sign of B-12 deficiency. A result higher than 70 pmol/l is normal. If it is between 25 and 70 pmol/l, an MMA test is prescribed.

A test for pernicious anaemia called Intrinsic Factor Blocking Antibody (IFBA) test detects low B-12 levels that result from the body’s inability to produce the intrinsic factor because of the anaemia.

What are the remedies?

Apart from increasing the intake of B-12 rich food, supplements are recommended. If absorption of the vitamin is problematic, oral supplementation may not be as effective. In such a situation, injections are preferred over tablets.

Since deficiency could spell serious interference with the functioning of the nervous system and the brain, it is better to get oneself tested and stay informed about the threat of any potential deficiency. At Apollo Diagnostics, we ensure proper testing procedures and quick results. Find an Apollo Diagnostics centre close to you.

Book Your Test

* T & C

You will get a call within 12 Hrs from the time of Booking. *T&C – Free home sample collection is not available on specific tests. Pediatric home sample collection ( kids below 10 years age ) is available at specific cities only

Patient Testimonials

“Finding the best place for a diagnostic test is as important as consulting the best doctor. The Apollo name was what guided me to Apollo Diagnostics and I am indeed glad that I came here. From attending us on time to sample collection, everything flowed so smoothly. I was particularly impressed by the hygiene. It was definitely a value for money experience. Thanks a lot Apollo Diagnostics…keep up the good work!”

Mrs. Ferha Jabeen, Old Bowenpally, Secunderabad

“I Have heard a lot of good things about Apollo Diagnostics from our family physician as well as from relatives and friends. So then next time I needed a diagnostic centre, I visited Apollo Diagnostics and I was really glad I did. For one, I didn’t have to wait. The technician was extremely good, and the place was really clean. It was certainly a value for money experience. Great job Apollo Diagnostics!”

Ms. T. Jaya, Nakkalagutta, Hanmakonda

“I must say Apollo Diagnostics places a great value on time and patient comfort! I am a fan of their home collection service which is not only on time and technically sound, but the hygiene and cordiality of the visiting technician is also commendable. Hats off! I’ll continue to recommend Apollo Diagnostics to my family and friends.”

Mr. B. Pochaiah, Mallikarjuna Nagar Colony, Old Bowenpally, Secunderabad

“I have always had a great experience with Apollo Diagnostics. The calls are unfailingly answered. The scheduled appointment is kept to the minute. The phlebotomist is well-trained in both technique and putting the patient at ease. Apollo Diagnostics undoubtedly delivers value for money. They will always be my top option for all the diagnostic needs of my family.”

Mr. Prasanta Kumar Jena, JP Nagar, Bengaluru

“I came to know about Apollo Diagnostics from my relatives and friends. So, when I required a check up to be done, I thought about trying their service once. From sticking to the appointed time to accuracy of technique, it was really a great experience overall. Hygiene of the technician and the centre as well, was also quite admirable. I certainly felt it delivers value for money service. From now on, I’ll always visit Apollo Diagnostics for all my checkups.”

Mr. Venkateshwar Rao, Hanmakonda

“I was looking for a good diagnostic centre to get some checkups done. I was confused as there are so many of them all over the city. Some of my friends recommended Apollo Diagnostics as the best one. I’ll always remain thankful to them. The technician at Apollo Diagnostics was not only punctual but his competency was certainly praiseworthy. I never felt any discomfort as he was so confident and compassionate. Great job Apollo Diagnostics!”

Mr. T Radhakishan Rao, Hanmakonda

“My experience with Apollo Diagnostics has been consistently good. Securing the appointment was very easy and the schedule was maintained to the dot. The behaviour of the technician was pleasant and he was well–versed with his job. The place was noticeably clean and I did not face any discomfort during the process. The reports were delivered as promised and surprisingly, the service was pretty affordable. I would recommend Apollo Diagnostics to my circle without any hesitation!”

Mr. Arif Ameer, JP Nagar, Bengaluru

“Apollo Diagnostics came highly recommended by my friends. I was particularly impressed by their cordiality, time management and process competence. Another must mention is the hygiene factor that is impeccable. Given the quality delivered, this is certainly a value for money experience. Thanks a lot Apollo Diagnostics…keep up the great work!”

Ms. A.Madhavi, Warangal

“Our family physician strictly instructed me to get the checkup done at Apollo Diagnostics. I am glad I respected his opinion. I was attended to as soon as I went in. The technician was a true expert at his job and made it a painless experience for me. I also observed that the centre was spotless and all the staff members were particular about maintaining hygiene. Apollo Diagnostics certainly delivers value for money. I’ll always recommend Apollo Diagnostics to my family and friends.”

Ms. Vimala, Hanmakonda

“My Apollo Diagnostics experience has thus far been truly amazing. Besides unfailingly maintaining the schedule – be it sample taking or report delivery, their sense of cleanliness is also tops. The technicians are well-trained and do their job with diligence and in a friendly manner that puts patients at ease at once. Apollo Diagnostics is setting a very high standard indeed! Now that’s what I call a fine job!”

Ms. G. Jyothi, Warangal

“My mother needed a blood test, but was unable to walk due to pain in her leg. A Phlebotomist from Apollo Diagnostics came home and collected the sample. I was informed, via SMS, that the report could be collected from any of the 3 centres in Dibrugarh, downloaded online or even delivered home for a nominal fee. Although the rates were reasonable, my mother also received a senior citizens discount too! This saved my time, money & energy. My aachhe din really seemed to have arrived! Thank you Apollo Diagnostics and hats off for displaying true professionalism.”

Mr. Alokk Kar Dowerah, Dibrugarh

“Whenever I need to have a blood sample collected from home, I ask for Vasu. He is punctual, courteous and so skilled I hardly feel any pain when he draws the sample. Plus, I truly appreciate how quickly the reports are delivered. Thanks Apollo!”

Mr. Sukumar, Film director, Sriram Nagar, Hyderabad